Avian sex determination is a DNA-based test that identifies the bird's sex by analyzing differences in sex chromosomes (Z and W). Male birds possess two identical sex chromosomes (ZZ), while females have two different ones (ZW). Highly conserved genetic sequences exist on these chromosomes across many bird species, and the difference between males and females can be detected using PCR amplification. In this process, females produce two DNA fragments of different sizes, while males yield a single fragment. This test is highly accurate and widely used for precise sex identification in birds of all ages and species.
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